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. 2017 Nov 29;8:966. doi: 10.3389/fphys.2017.00966

Figure 3.

Figure 3

Influence of MiR-29a and TRPV4 on GC-1 cell apoptosis in vitro (A) qRT-PCR assays were performed to analyze the expression level of miR-29a after transfection with pri-miR-29a or anti-miR-29a in 3 h OGD/24 h reoxygenation treatments. *p < 0.05 vs. non-trans, n = 6 per group; (B,C) Flow cytometry assays were performed to show the cell apoptosis after transfection with pri-miR-29a or anti-miR-29a in normoxia and 3 h OGD/24 h reoxygenation treatments. *p < 0.05 vs. non-trans, n = 6 per group; (D–K) Cleaved caspase-3, Bax and Bcl-2 protein levels transfected with pri-miR-29a, anti-miR-29a, TRPV4 siRNAs, or TRPV4-overexpression(GC-1/TRPV4) were examined in normoxia and 3 h OGD/24 h reoxygenation treatments by western blot analysis. *p < 0.05 vs. non-trans, n = 6 per group.