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. 2017 Nov 29;8:966. doi: 10.3389/fphys.2017.00966

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Copyright © 2017 Ning, Li, Cheng, Yu, Rao, Ruan, Yuan, Zhang, Zhuo, Du and Xiao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MiR-29a inhibits apoptosis of testicular IRI via downregulation of TRPV4 in vivo. (A,B) TUNEL assays were performed to investigate the cell apoptosis after injection with miR-29a agomir and its negative ctrl at 16 h of reperfusion after 1 h ischemia. ^*p < 0.05 vs. none(1 h ischemia/16 h reperfusion treatment), n = 5 per group; (C) Immunohistochemistry staining of Bax and Bcl-2 after injection with miR-29a agomir and its negative ctrl at 1 h of ischemia/16 h of reperfusion. n = 5 per group; (D–F) TRPV4 and Cleaved caspase-3 protein level expression after injection with miR-29a agomir and its negative ctrl were detected at 1 h of ischemia followed by 16 h of reperfusion by western blot analysis. ^*p < 0.05 vs. none, n = 5 per group.