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Number (n) of monovalent (K+) and divalent (Mg2+) cations released with cofilin binding to actin filaments determined from the salt concentration dependence of the intrinsic binding constant (Ka) and cooperativity parameter (ω)
Uncertainties are from linear fits (Fig. 3).
| Cofilin | n (Ka) | n (ω) |
|---|---|---|
| S3D | 2.3 ± 0.7 K+ | ∼0 |
| 0.9 ± 0.1 Mg2+ | ∼0 | |
| WT | 1.7 ± 0.2 K+ | ∼0 |
| 0.7 ± 0.1 Mg2+ | ∼0 |
