Figure 7.

Effects of site-specific mutations of Pah1 within the sequence WRDPLVDID on the complementation of the pah1Δ temperature-sensitive phenotype. The indicated wild-type and site-specific mutant forms of Pah1 were expressed from pGH315-based plasmids (Table 1) in the pah1Δ mutant strain SS1026. The cells were grown to saturation in SC−Leu medium at 30 °C; serial dilutions (10-fold) of the cells were spotted onto SC−Leu agar plates, and growth was scored after 3 days of incubation at 30 and 37 °C. The data are representative of three independent experiments.