Figure 3.
CpaA aids in colonization of the spleen in mice. Mice were intranasally inoculated with 1 × 109 cfus of either wild-type, ΔcpaA, or the cpaA complemented strain. 36 h post-infection, mice were sacrificed, organs were harvested, and colony-forming units were enumerated from homogenized tissue. A, total colony-forming units from the lung demonstrate a non-statistically significant trend toward decreased colonization by the ΔcpaA strain (Kruskal-Wallis non-parametric, p = 0.06). B, total colony-forming units from the spleen demonstrate a statistically significant difference between the complemented cpaA strain and the ΔcpaA mutant strain (Kruskal-Wallis non-parametric, *p = 0.0327).