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The C-terminal domain of CpaB is exposed to the periplasmic space. Shown is a Western blot analysis probing for RNA polymerase as a cytoplasmic control and CpaB-His of samples treated with 0.5 μg/ml proteinase K for 0–20 min at 56 °C. A, limited proteolysis of M2 whole cells expressing pWH-cpaB-his. B, limited proteolysis of spheroplasts isolated from M2 cells expressing pWH-cpaB-his.
