Figure 3.

GH and OSM induce the interaction between STAT5A and PDC-E2 in a time-dependent manner. Fully differentiated 3T3-L1 adipocytes were treated with mGH or OSM for the indicated times. Monolayers were scraped into IP lysis buffer, and whole-cell lysates were prepared. IP experiments were performed using the anti-STAT5A antibody and 300 μg of lysate protein. The mock experiment contained IP antibody but no lysate. Western blotting was used to examine the protein content of the immunoprecipitates (IP: STAT5A; left) and lysate inputs (Direct WB; right). This experiment was repeated three times on independent batches of adipocytes.