Figure 4.

PDC subunits differentially interact with members of the STAT protein family. Fully differentiated 3T3-L1 adipocytes were treated with vehicle (V) (0.1% BSA/PBS) or 1 nm OSM for 20 min, a condition that activates STAT5, STAT3, and STAT1. Monolayers were scraped into IP lysis buffer, and whole-cell lysates were prepared. IP reactions were performed using antibodies targeted against the indicated STAT proteins and 300 μg of lysate protein. The mock experiment contained IP antibody but no lysate. Western blotting was used to examine the protein content of the immunoprecipitates (IPs; left) and lysate inputs (Direct WB; right). This experiment was repeated at least three times on independent batches of cells.