Figure 4.

Incorporation of Kdo and Kdo-N₃ into LPS of ClearColi. ClearColi K-12 was grown in LB in the presence or absence of 5 mm Kdo or Kdo-N₃ and labeled with Alexa488-DIBO. The whole-cell extracts were separated using SDS-PAGE. A, Alexa Fluor 488-labeled LPS in SDS-polyacrylamide gel visualized by fluorescent imaging; B, SDS-polyacrylamide gel after staining LPS with the Pro-Q Emerald 300 LPS staining kit. The data are representative of three independent biological replicates.