Table 1.
Observed lipid IVA species in total lipid extracts of ClearColi K-12 evaluated by MALDI-TOF-MS
The data are representative of two independent experiments.
| LPS species | Calculated mass [M − H]− (m/z) | Observed mass [M − H]− (m/z) | Relative signal intensity (%) |
|---|---|---|---|
| ClearColi | |||
| Lipid IVA | 1403.85 | 1403.80 | 100 |
| Kdo-lipid IVA | 1623.90 | 1623.83 | 0.11 |
| Kdo-N3-lipid IVA | 1648.91 | NDa | ND |
| (Kdo)2-lipid IVA | 1843.96 | ND | ND |
| Kdo-Kdo-N3-lipid IVA | 1868.97 | ND | ND |
| (Kdo-N3)2-lipid IVA | 1893.98 | ND | ND |
| ClearColi grown with 1 mm Kdo-N3 | |||
| Lipid IVA | 1403.85 | 1403.80 | 100 |
| Kdo-lipid IVA | 1623.90 | 1623.83 | 0.10 |
| Kdo-N3-lipid IVA | 1648.91 | 1648.84 | 0.48 |
| (Kdo)2-lipid IVA | 1843.96 | ND | ND |
| Kdo-Kdo-N3-lipid IVA | 1868.97 | ND | ND |
| (Kdo-N3)2-lipid IVA | 1893.98 | 1893.89 | 0.02 |
a ND, not detected.