Table 4.
Populations of the FBP cleavage product (triose-Ps) at varying local FBP concentrations in class I aldolase
| [FBP] | Percentage of product as triose-Pa |
|
|---|---|---|
| Keq = 10−4 mb | Keq = 10−5 m | |
| μm | % | % |
| 1 | 99.0 | 91.6 |
| 10 | 91.6 | 61.8 |
| 100 | 61.8 | 27.0 |
| 1000 | 27.0 | 9.5 |
a Theoretical populations were calculated for the Keq range (10−4 to 10−5 m) reported in the literature.
b Keq, equilibrium constant for the aldolase cleavage reaction. Equation 3 (see “Experimental procedures”) was used to calculate the population of species.