Figure 4.

Lys-714 does not regulate the general stability of ErbB4. A, COS-7 cells expressing wild-type or K714R ErbB4 JM-a CYT-2 were starved without serum overnight and treated with 100 μg/ml cycloheximide (CHX) for the indicated periods of time. Lysates were analyzed by Western blotting with anti-ErbB4, and equal loading was controlled with anti-actin. B, quantification of 180 kDa or 75 kDa ErbB4 signal intensities (shown in A) normalized to actin. Data are represented as mean ± range from three independent experiments.