Table 3.
Delimitation of structural “fingers” of the two catalytic modules of κ-carrageenases from Z. galactanivorans (5OCR) and P. carrageenovora, mutant E168D in complex with κ-neocarratetraose (5OCQ) and also the apoenzyme (1DYP), and associated values of B factor
B factor values that vary significantly from values of the full sequence are underlined.
| Finger | ZgCgkAGH16 | B factor | PcCgkAGH16-E168D | B factor | PcCgkAGH16 | B factor |
|---|---|---|---|---|---|---|
| Full sequence | 26.0 | Full sequence | 12.6 | Full sequence | 10.3 | |
| F1 | Trp62–Trp76 | 30.8 | Trp57–Trp69 | 6.2 | Trp57–Trp69 | 8.3 |
| F2 | Arg92–Tyr106 | 11.3 | Arg92–Tyr106 | 12.4 | ||
| F3 | Asp145–Val156 | 24.9 | Thr148–Gln160 | 22.4 | Thr148–Gln160 | 12.8 |
| F4 | Phe168–Asp177 | 32.3 | ||||
| F5 | Lys190–Lys201 | 28.5 | Val187–Gly198 | 25.3 | Val187–Gly198 | 14.6 |
| F6 | Lys264–Ala276 | 49.6 | Ala261–Pro273 | 11.2 | Ala261–Pro273 | 9.1 |