Figure 2.

ARV drugs regulate astrocyte ER stress markers and AEG-1 mRNA levels. Astrocytes were treated with a panel of drugs, including NRTIs (abacavir, lamivudine, and stavudine), NNRTIs (nevirapine and delavirdine), and PIs (darunavir, lopinavir, and ritonavir) with CPE between 1 and 4 at therapeutic doses along with vehicle controls for 8 h. PERK (a), ATF6 (b), BiP (c), and AEG-1 (d) mRNA levels were measured using RT²-PCR. Fold changes to untreated control were calculated using GAPDH as a normalizing control. Cumulative data from a minimum of three independent donors are shown. Data represent mean±S.E.M., and statistical analyses were performed using one-way ANOVA with Tukey’s post-test for multiple comparisons (*P<0.05, **P<0.01, ***P<0.001).