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. 2017 Dec 4;3:17061. doi: 10.1038/cddiscovery.2017.61

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Copyright © 2017 The Author(s)

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IL-1β and abacavir activate UPR and trigger eIF2α phosphorylation. Astrocytes were treated with IL-1β (20 ng/ml) or abacavir (4 μM) for 15 and 30 min. Total protein lysates were immunoblotted for p-eIF2α and eIF2α (a). Densitometry analyses were performed to quantify the intensity ratio of p-eIF2α to total eIF2α, which are graphed as fold change to control. One-way ANOVA with Tukey’s post-test was used to determine statistical significance (*P<0.05). Whole-cell lysates of astrocytes treated with IL-1β (20 ng/ml) for 6 h were immunoblotted for ATF4 (b), XBP-1s (c), and ATF6 (d). Statistical analyses were performed by Student’s unpaired T-test to compare fold change to control (***P<0.001). In all panels, representative blots are presented, while the average fold change from three independent donors is graphed. GAPDH was used as a normalizing loading control.