Fig. 7.

Intracellular Ca²⁺ increased in response to hypoxia in isolated neuroepithelial cells (NECs) of goldfish. A: representative recording of a NEC responding to hypoxia by increasing intracellular Ca²⁺ concentration ([Ca²⁺]_i). Upper trace shows 340/380-nm excitation ratio (R_340/380) using fura-2 AM; bottom traces show raw intensities at each wavelength. B: Plot of means ± SE R_340/380 indicates that [Ca²⁺]_i rose significantly during hypoxia compared with normoxia (*P < 0.01, Wilcoxon matched-pairs test; n = 7).