Table 1.
List of primers and assays for PCR analysis and transfection experiments.
| Primer sequences | ||
|---|---|---|
| Gene | Sequence forward | Sequence reverse |
| ACAN | TCGAGGACAGCGAGGCC | TCGAGGGTGTAGCGTGTAGAGA |
| ALPL | CCACGTCTTCACATTTGGTG | GCAGTGAAGGGCTTCTTGTC |
| COL10A1 | TGCTGCCACAAATACCCTTT | GTGGACCAGGAGTACCTTGC |
| COL1A1 | CCTGGATGCCATCAAAGTCT | CGCCATACTCGAACTGGAAT |
| COL2A1 variant B | CAGACGCTGGTGCTGCT | TCCTGGTTGCCGGACAT |
| COL3A1 | CGCCCTCCTAATGGTCAAGG | AGGGCCTGAAGGACCAGCTT |
| COX2 | CGGTGAAACTCTGGCTAGACAG | GCAAACCGTAGATGCTCAGGGA |
| HAPLN1 | TTCCACAAGCACAAACTTTACACAT | GTGAAACTGAGTTTTGTATAACCTCTCAGT |
| IDO1 | GCCTGATCTCATAGAGTCTGGC | TGCATCCCAGAACTAGACGTGC |
| IL6 | AGACAGCCACTCACCTCTTCAG | TTCTGCCAGTGCCTCTTTGCTG |
| IL8 | GAGAGTGATTGAGAGTGGACCAC | CACAACCCTCTGCACCCAGTTT |
| MMP13 | GACTTCCCAGGAATTGGTGA | TACCCCAAATGCTCTTCAGG |
| RSP9 | ATGAAGGACGGGATGTTCAC | GATTACATCCTGGGCCTGAA |
| SOX9 | AGGTGCTCAAAGGCTACGAC | GTAATCCGGGTGGTCCTTCT |
| THBS5 | CCGACAGCAACGTGGTCTT | CAGGTTGGCCCAGATGATG |
| TNFα | AGCCCATGTTGTAGCAAACCCTC | TGGTTATCTCTCAGCTCCACGCCA |
| TSG6 | TCACCTACGCAGAAGCTAAGGC | TCCAACTCTGCCCTTAGCCATC |
| TaqMan® Gene Expression Assay ID | ||
| Gene | ID | |
| ARG1 | Hs00163660_m1 | |
| COL4A1 | Hs01098873_m1 | |
| CSTA | Hs00193257_m1 | |
| EDIL3 | Hs00174781_m1 | |
| IGFBP5 | Hs00181213_m1 | |
| NDRG1 | Hs00608387_m1 | |
| PTX3 | Hs00173615_m1 | |
| PYCARD | Hs01547324_m1 | |
| RPS9 | Hs02339424_m1 | |
| SRGN | Hs01004159_m1 | |
| THBS1 | Hs00962908_m1 | |
| THBS2 | Hs01568063_m1 | |
| THBS3 | Hs00938498_m1 | |
| THBS4 | Hs00170261_m1 | |
| UCHL3 | Hs00234683_m1 | |
| XYLT1 | Hs00544498_m1 | |
| siRNA references | ||
| Target | ID | |
| siCT | Ambion®, 4390844 | |
| siTHBS1 | Ambion®, s14098 | |
THBS1, thrombospondin-1.