Table 2.
Proteins identified in the secretome of chondrocyte/adipose stem cells (ASC) cocultures.
| Gene name | Number of quantified peptides | Protein coverage (%) | Median score of coculture | Median score of mix | Ratio coculture/mix |
|---|---|---|---|---|---|
| PYCARD | 5 | 16.4 | 361 | 131 | 2.76 |
| CSTA | 5 | 39.8 | 245 | 100 | 2.45 |
| SRGN | 5 | 10.1 | 230 | 110 | 2.09 |
| XYLT1 | 9 | 3.4 | 238 | 130 | 1.83 |
| THBS1 | 33 | 22.0 | 1,343 | 759 | 1.77 |
| PTX3 | 22 | 41.5 | 1,341 | 841 | 1.59 |
| EDIL3 | 9 | 13.8 | 356 | 233 | 1.53 |
| UCHL3 | 6 | 24.4 | 128 | 192 | 0.67 |
| IGFBP5 | 15 | 40.8 | 260 | 406 | 0.64 |
| COL4A2 | 9 | 2.9 | 122 | 199 | 0.61 |
| NDRG1 | 6 | 4.1 | 67 | 112 | 0.60 |
| ARG1 | 7 | 5.6 | 43 | 139 | 0.31 |
Secretome analysis compared proteins produced in the supernatants from chondrocyte/ASC coculture versus supernatants from either monoculture or mixed supernatants from both monocultures. Among 2043 identified proteins by MS/MS, 92 were significantly differentially secreted in chondrocyte/ASC cocultures as compared to mixed monocultures and only 12 were selected for validation.
PYCARD, PYD and CARD domain containing; CSTA, cystatin A; SRGN, serglycin; XYLT1, xylosyltransferase 1; THBS1, thrombospondin-1; PTX3, pentraxin-3; EDIL3, EGF-like repeats and discoidin domains 3; UCHL3, ubiquitin C-terminal hydrolase L3; IGFBP5, insulin-like growth factor binding protein 5; COL4A2, collagen type IV alpha 2; NDRG1, N-Myc downstream regulated 1; ARG1, arginase 1.