Table 1.
Curated list of candidate interactors found in untargeted yeast 2-hybrid library screens.
| Gene | Description | MW (kDa) | TM domains | Location | CYP83A1/Node | CYP83A1/Hypocotyl | CYP83B1/Node | CYP83B1/Hypocotyl |
|---|---|---|---|---|---|---|---|---|
| At1G04750.1 | Vesicle associated membrane protein 721 (VAMP721) | 24.8 | 1 | Cell membrane | 1 | – | – | – |
| At1G06090.1 | Fatty acid desaturase family | 34.8 | 5 | ER membrane | – | – | 1 | – |
| At1G15500.1 | ATP:ADP antiporter (NTT2) | 67.5 | 11 | Plastid membrane | – | – | 1 | – |
| At1G16890.2 | Ubiquitin-conjugating enzyme 36 (UBC36) | 17.2 | na | 1 | – | – | – | |
| At1G26670.1 | Vesicle transport V-SNARE 12 (VTI12) | 25.1 | 1 | Golgi network membrane | 1 | – | – | – |
| At1G28250.1 | Unknown | 11.2 | 2 | na | – | – | 1 | – |
| At1G28490.1 | Syntaxin of plants 61 (SYP61) | 27.7 | 1 | Golgi network membrane | 2 | – | – | – |
| At1G30935.1 | F-box protein family (FBX1) | 46.5 | 1 | Membrane | 1 | – | – | – |
| At1G44920.1 | Unknown | 27.7 | 4 | na | 1 | – | 1 | – |
| At1G52260.1 | Protein disulfide isomerase-like, thioredoxin superfamily (PDI3) | 60.2 | ER | – | – | 1 | – | |
| At1G52870.2 | Mpv17/PMP22 family; Peroxisomal membrane protein | 41.3 | Chloroplast | – | – | 1 | – | |
| At1G57765.1 | Unknown | 11.9 | 1 | Secreted | – | – | 1 | 2 |
| At1G59890.2 | SIN3-LIKE 5 (SNL5) | 133.0 | Nucleus | – | – | 1 | – | |
| At1G66240.1 | Homolog of anti-oxidant 1 (ATX1) | 8.2 | Cytosol | – | – | 1 | – | |
| At1G68220.1 | Unknown | 21.4 | 4 | Secreted | 1 | – | - | – |
| At2G05630.1 | ATG8D | 13.9 | Cytoplasmic vesicle, Lipid anchor | 1 | – | – | – | |
| At2G25610.1 | ATPase, F0/V0 complex, subunit C protein; | 18.2 | 4 | ER membrane | 2 | 1 | – | – |
| At2G28900.1 | Outer envelope protein 16 (OEP16) | 15.5 | 3 | Plastid outer membrane | 1 | – | – | – |
| At2G32380.1 | Transmembrane protein 97 | 18.3 | 4 | Secreted | 1 | – | – | – |
| At2G32720.1 | Cytochrome B5-B (CB5-B) | 15.0 | 1 | ER membrane | 1 | – | – | – |
| At2G33120.1 | Vesicle associated membrane protein 722 (VAMP722) | 24.9 | 1 | Cell membrane | 2 | – | – | – |
| At2G41110.1 | Calmodulin 2, AtCAL5 (CAM2) | 16.8 | Cytoplasm, cytoskeleton | 1 | – | – | – | |
| At3G12120.1 | Fatty acid desaturase 2 (FAD2) | 44.0 | 5 | ER membrane | 1 | 1 | – | – |
| At3G12870.1 | Unknown | 23.7 | 3 | Other (e.g., cytoplasm) | – | – | 1 | – |
| At3G13520.1 | Arabinogalactan protein 12 (AGP12) | 6.1 | Cell membrane (GPI-anchor) | – | – | 1 | – | |
| At3G15820.1 | Reduced oleate desaturation 1, phosphatidylcholine:diacylglycerol cholinephosphotransferase (ROD1) | 33.0 | 5 | ER membrane | 1 | – | – | – |
| At3G18280.1 | Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein | 10.3 | Secreted | – | – | – | 1 | |
| At3G48990.1 | Acyl-activating enzyme 3 (AAE3) | 55.5 | Cytoplasm | – | – | – | 1 | |
| At3G50685.1 | Unknown | 16.1 | 3 | na | – | – | 1 | – |
| At3G51460.1 | Root hair defective 4 (RHD4) | 68.2 | 2 | ER membrane | – | 1 | – | – |
| At3G60600.1 | VAMP/Synaprobrevin-associated protein 27-1, Vesicle associated protein (VAP27-1) | 28.5 | 1 | ER membrane | – | 1 | – | – |
| At4G01150.1 | Curvature thylakoid 1A (CURT1A) | 17.7 | 2 | Plastid, chloroplast | 1 | – | – | – |
| At4G14420.1 | HR-like lesion-inducing protein-related | 17.4 | 2 | Secreted | 2 | 1 | – | – |
| At4G16410.1 | Unknown | 20.4 | 3 | na | 4 | – | 1 | – |
| At4G24920.1 | Sec61 subcomplex, protein transmembrane transporter | 7.7 | 1 | ER membrane | 1 | – | 2 | – |
| At4G27500.1 | Proton pump interactor 1 (PPI1) | 68.9 | 1 | Cell membrane | – | 3 | – | – |
| At4G30950.1 | Fatty acid desaturase 6 (FAD6) | 51.2 | 4 | Plastid inner membrane | – | – | 1 | – |
| At4G31840.1 | Early nodulin-like protein 15 (ENODL15) | 19.0 | 1 | Extracellular | 4 | 3 | 7 | 3 |
| At5G14330.1 | Unknown | 14.2 | 2 | na | 1 | – | – | – |
| At5G14720.1 | Protein kinase superfamily | 75.4 | Other (e.g., cytoplasm) | – | – | 1 | – | |
| At5G16830.1 | Syntaxin of plants 21 (SYP21) | 31.1 | 1 | Prevacuolar compartment | – | – | – | 2 |
| At5G20130.1 | Unknown | 21.9 | na | 1 | – | – | – | |
| At5G24680.1 | Peptidase C78, ubiquitin fold modifier-specific peptidase 1/ 2 | 44.3 | na | 4 | – | – | – | |
| At5G38660.1 | Acclimation of photosynthesis to environment (APE1) | 31.4 | 1 | Plastid | – | – | 1 | – |
| At5G39510.1 | Vesicle transport V-SNARE 11 (VTI11) | 25.0 | 1 | Golgi network membrane | 1 | – | – | – |
| At5G42000.1 | ORMDL family protein | 18.1 | 3 | Other (e.g., cytoplasm) | 2 | – | – | – |
| At5G42570.1 | B-cell receptor-associated 31-like | 24.6 | 3 | Secreted | – | – | 1 | – |
| At5G43460.1 | HR-like lesion-inducing protein-related | 16.9 | 3 | Secreted | 63 | 6 | 17 | 6 |
| At5G43580.1 | Unusual serine protease inhibitor (UPI) | 11.1 | na | – | – | – | 1 | |
| At5G43970.1 | Translocase of outer membrane 22-V, TOM9-2 (TOM22-V) | 10.4 | 1 | Mitochondrion outer membrane | – | – | 5 | - |
| At5G45420.1 | Membrane anchored MYB (maMYB) | 34.4 | 2 | Other (e.g., cytoplasm) | 1 | 1 | 2 | 1 |
| At5G45680.1 | FK506 binding protein 13, peptidyl-prolyl isomerase (FKBP13) | 22.0 | Plastid | – | 1 | – | – | |
| At5G50740.3 | Heavy metal transport/detoxification superfamily protein | 32.9 | Other (e.g. cytoplasm) | – | – | – | 1 | |
| At5G51010.1 | Rubredoxin-like superfamily | 17.2 | 1 | na | 2 | – | 1 | – |
| At5G51400.1 | PLAC8 family | 27.0 | Other (e.g., cytoplasm) | – | – | – | 1 | |
| At5G52240.1 | Membrane-associated progesterone binding protein 5, ATMP1, Membrane steroid binding protein 1 (MSBP1) | 24.4 | 1 | Cell membrane | – | 1 | – | – |
| At5G52980.1 | ATPase, vacuolar ER assembly factor, Vma12 | 24.5 | 2 | na | – | – | 1 | – |
| At5G60920.1 | COBRA (COB) | 51.2 | Cell membrane, Lipid anchor | 1 | – | – | – | |
| At5G61790.1 | Calnexin 1 (CNX1) | 60.5 | 1 | ER membrane | 1 | – | – | – |
| At5G67600.1 | Windhose 1 (WIH1) | 8.7 | Other (e.g., cytoplasm) | 4 | – | – | – |
Gene models and descriptions are based on The Arabidopsis Information Resource (TAIR10, www.arabidopsis.org). Information about the molecular weight (MW) and transmembrane (TM) domains were obtained from the UniProt databases (Bateman et al., 2015; Boutet et al., 2016). Information and predictions of protein locations are based on UniProt databases (regular font) or the SUBA3 database (italic font) (Hooper et al., 2014). The columns to the right indicate occurrences of sequenced plasmids containing the respective sequences in the four screens with combinations of CYP83A1 or CYP83B1 as baits and libraries constructed from node and hypocotyl tissue. na, not available.