Table 2.
Curated list of candidate interactors found by Co-IP with CYP83A1-mVenus.
| Gene | Description | MW (kDa) | TM domains | Location | Exp1 | Exp2 | Exp3 | Total specs |
|---|---|---|---|---|---|---|---|---|
| At1G11750 | CLP protease proteolytic subunit 6 (CLPP6) | 29.4 | Plastid, stroma | 2 | 0 | 5 | 7 | |
| At1G14810 | Semialdehyde dehydrogenase family protein | 40.7 | Plastid | 2 | 0 | 4 | 6 | |
| At1G20580 | Small nuclear ribonucleoprotein family protein (SMD3) | 14.2 | Cytosol | 7 | 4 | 0 | 11 | |
| At1G54100 | Aldehyde dehydrogenase 7B4 (ALDH7B4) | 54.2 | Cytosol | 1 | 0 | 5 | 6 | |
| At1G68830 | STT7 homolog (STN7) | 63.3 | Plastid, thylakoid membrane | 0 | 0 | 5 | 5 | |
| At1G70730 | Phosphoglucomutase/phosphomannomutase family protein (PGM2) | 63.5 | Cytoplasm | 3 | 0 | 4 | 7 | |
| At2G18960 | H(+)-ATPase 1 (HA1) | 104.2 | 10 | Cell membrane | 3 | 0 | 3 | 6 |
| At2G40490 | Uroporphyrinogen decarboxylase (HEME2) | 43.6 | Plastid | 2 | 0 | 3 | 5 | |
| At3G09200 | Ribosomal protein L10 family protein | 34.1 | Cytosol | 6 | 0 | 6 | 12 | |
| At3G19170 | Presequence protease 1 (PREP1) | 121.0 | Plastid, stroma, Mito., matrix | 6 | 1 | 7 | 14 | |
| At3G22142 | Encodes a Protease inhibitor/seed storage/LTP family protein | 147.2 | Secreted | 8 | 0 | 0 | 8 | |
| At3G26070 | Plastid-lipid associated protein PAP / fibrillin family protein; | 27.2 | Plastid | 0 | 0 | 6 | 6 | |
| At3G48000 | Aldehyde dehydrogenase 2 (ALDH2) | 58.6 | Mitochondrion, matrix | 5 | 0 | 10 | 15 | |
| At3G48420 | Haloacid dehalogenase-like hydrolase superfamily protein | 34.2 | Plastid | 5 | 0 | 1 | 6 | |
| At3G56240 | Copper chaperone (CCH) | 13.0 | Cytosol | 4 | 2 | 2 | 8 | |
| At3G57610 | Adenylosuccinate synthase (ADSS) | 53.0 | Plastid | 3 | 0 | 3 | 6 | |
| At3G58730 | Vacuolar ATP synthase subunit D (VATPD) | 29.1 | Vacuole membrane | 0 | 0 | 5 | 5 | |
| At3G61440 | Cysteine synthase C1 (CYSC1) | 39.9 | Mitochondrion | 5 | 2 | 8 | 15 | |
| At4G13770 | Cytochrome P450, family 83, subfamily A, polypeptide 1 (CYP83A1) | 57.4 | 1 | ER membrane | 157 | 37 | 34 | 228 |
| At4G15530 | Pyruvate orthophosphate dikinase (PPDK) | 105.1 | Cytosol, Plastid | 0 | 0 | 7 | 7 | |
| At4G22240 | Plastid-lipid associated protein PAP / fibrillin family protein | 33.7 | Plastid | 1 | 0 | 5 | 6 | |
| At4G23100 | Glutamate-cysteine ligase (GSH1) | 58.6 | Plastid | 3 | 0 | 3 | 6 | |
| At4G23600 | Tyrosine transaminase family protein (CORI3) | 47.0 | Cytosol | 0 | 2 | 11 | 13 | |
| At4G25080 | Magnesium-protoporphyrin IX methyltransferase (CHLM) | 33.8 | Plastid, peripheral membrane | 1 | 0 | 5 | 6 | |
| At4G30270 | Xyloglucan endotransglucosylase/hydrolase 24 (XTH24) | 30.8 | Secreted | 0 | 0 | 5 | 5 | |
| At4G33680 | Pyridoxal phosphate-dependent transferases superfam. prot. (AGD2) | 50.4 | Plastid | 3 | 0 | 2 | 5 | |
| At5G11520 | Aspartate aminotransferase 3 (ASP3) | 49.0 | Plastid | 1 | 0 | 4 | 5 | |
| At5G11670 | NADP-malic enzyme 2 (NADP-ME2) | 64.4 | Cytoplasm | 5 | 0 | 1 | 6 | |
| At5G14200 | Isopropylmalate dehydrogenase 1 (IMD1) | 44.2 | Plastid | 3 | 0 | 5 | 8 | |
| At5G14780 | Formate dehydrogenase (FDH) | 42.4 | Mitochondrion | 6 | 1 | 7 | 14 | |
| At5G17710 | Co-chaperone GrpE fam. protein/embryo defective 1241 (EMB1241) | 35.5 | Mitochondrion, matrix | 6 | 1 | 4 | 11 | |
| At5G19550 | Aspartate aminotransferase 2 (ASP2) | 44.3 | Cytoplasm | 1 | 0 | 5 | 6 | |
| At5G38660 | Acclimation of photosynthesis to environment (APE1) | 31.4 | 1 | Plastid | 8 | 0 | 1 | 9 |
| At5G42650 | Allene oxide synthase (AOS) | 58.2 | Plastid | 6 | 0 | 15 | 21 | |
| At5G44130 | FASCICLIN-like arabinogalactan protein 13 precursor (FLA13) | 26.2 | Cell membrane, Lipid anchor | 2 | 0 | 3 | 5 | |
| At5G49360 | Beta-xylosidase 1 (BXL1) | 83.5 | Secreted | 0 | 0 | 11 | 11 | |
| At5G51820 | Phosphoglucomutase (PGM1) | 68.0 | Plastid | 6 | 2 | 9 | 17 | |
| At5G60600 | 4-hydroxy-3-methylbut-2-enyl diphosphate synthase (HDS) | 82.3 | Plastid, stroma | 1 | 0 | 10 | 11 |
Gene identifiers and descriptions are based on The Arabidopsis Information Resource (TAIR10, www.arabidopsis.org). Information about the molecular weight (MW) and transmembrane (TM) domains were obtained from the UniProt databases (Bateman et al., 2015; Boutet et al., 2016). Information and predictions of protein locations are based on UniProt databases (regular font) or the SUBA3 database (italic font) (Hooper et al., 2014). Columns Exp and total specs show the number of recorded spectra in each of the three experiments or the sum thereof, respectively.