Figure 3.

(A) Traces of [Ca²⁺]_i transients of a neuron (red trace) and a mast cell (blue trace) in response to nerve stimulation (EFS). Neuronal responses occurred instantaneously with the stimulus, while MC activation occurred after a time delay. Both, neuronal responses and mast cell activation were reproducible (compare solid and dotted traces). There were no differences between 1st and 2nd EFS evoked MC signaling regarding amplitude of [Ca²⁺]_i peaks (B), time point of the [Ca²⁺]_i peak (C) or percentage of responding mast cells (D). P-values based on Wilcoxon Signed Rank Test. Results based on 13 tissues, 13 ganglia, and 78 mast cells.