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. 2017 Nov 29;8:971. doi: 10.3389/fphys.2017.00971

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Copyright © 2017 Buhner, Barki, Greiter, Giesbertz, Demir, Ceyhan, Zeller, Daniel and Schemann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Mast cell activation (blue trace) evoked by nerve stimulation (EFS) (red trace) was completely blocked by the nerve blocker tetrododoxin (TTX) (middle panel) and returned after TTX washout (lower panel). (B) Correspondingly, the amplitude of the [Ca²⁺]_i signal (upper panel), the percentage of responding mast cells (middle panel) and the mast cell response index (lower panel) were significantly reduced by TTX. P-values based on Friedman analysis of variance on ranks and post-hoc Tukey test; results based on 6 tissues, 6 ganglia, and 39 mast cells.