Table 3.
The concentration of cytokines in supernatants of HBMLs after 24 h of co-cultivation with nanoparticles of iron oxides at various doses, Me (Q1–Q3).
| MTD | The Concentration of Cytokines, pg/mL, n = 3 | |||||||
|---|---|---|---|---|---|---|---|---|
| IL-2 | IL-4 | IL-6 | IL-8 | IL-10 | GM-CSF | IFNγ | TNFa | |
| 0 | 4.23 (3.87–4.51) |
1.26 (0.73–1.98) |
49.0 (43.5–54.3) |
2345 (2189–2416) |
6.27 (5.99–8.55) |
10.61 (10.23–13.40) |
66.2 (59.1–68.9) |
48.3 (44.3–56.4) |
| 2.3 FF1 |
6.50 (6.23–6.56) |
1.82 (1.70–1.86) |
56.3 (55.7–57.0) |
3560 (3507–3651) |
14.28 (13.2–15.32) |
17.83 (16.22–18.21) |
97.6 (95.4–100.2) |
70.0 (69.3–70.2) |
| FF2 |
9.20 * (9.03–10.11) |
2.65 * (2.57–2.76) |
58.0 * (57.7–58.5) |
4598 * (4570–4610) |
17.56 * (16-09–18-21) |
25.6 * (24.2–26.0) |
137.7 * (135.2–138.2) |
165.2 * (152.3–167.4) |
| 10 FF1 |
10.04 (9.8–11.2) |
2.92 (2.82–3.01) |
60.6 (60.1–61.0) |
4494 (4489–4500) |
52.74 (48.12–56.33) |
26.88 (25.3–26.9) |
146 (132.8–147.5) |
216.4 (210.2–219.0) |
| FF2 | 6.19 * (6.09–7.11) |
1.85 * (1.8–1.95) |
55.6 * (55.0–56.1) |
2905 * (2800–3100) |
10.4 * (10.18–11.1) |
25.9 * (25.53–26.33) |
110.1 * (108.2–112.6) |
229.3 * (220.3–230.3) |
| 100 FF1 |
12.8 (12.08–13.01) |
3.42 (3.32–3.56) |
63.1 (62.5–63.4) |
4778 (4770–4790) |
267.6 (259.3–280.9) |
30.12 (29.11–31.24) |
197.7 (189.0–2017) |
278.3 (262.0–280.7) |
| FF2 | 3.8 * (3.63–4.01) |
1.35 * (1.29–1.4) |
50.7 * (50.0–51.0) |
2900 * (2895–3000) |
5.96 * (4.91–6.11) |
16.92 * (16.8–17.22) |
79.5 * (78.1–81.1) |
78.3 * (76.2–81.8) |
| 1000 FF1 |
14.23 (13.12–14.98) |
3.82 (3.75–4.01) |
62.6 (62.1–63.0) |
4998 (4980–5000) |
295.5 (285.3–302.3) |
31.76 (30.12–32.12) |
200.9 (198.9–202.2) |
318.2 (315.2–323.4) |
| FF2 | 10.11 * (9.4–11.01) |
2.98 * (2.81–3.01) |
60.6 * (60.0–60.9) |
5280 * (5257–5310) |
60.8 * (58.2–61.1) |
39.79 * (37.8–40.22) |
161.6 * (159.8–162.1) |
239.2 * (232.3–246.5) |
Note: n is the number of samples tested in each group. Almost all values at doses 2.3–1000 MTD (bold font) are above the corresponding control samples (0 MTD); (*)—values for FF2 suspension are higher or lower than corresponding value for FF1 suspension without chitosan; p < 0.05 according to the Mann-Whitney test.