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. 2017 Dec 4;11:37. doi: 10.1186/s13036-017-0076-1

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — TEER and permeability measurements for assessing barrier function. (a) The transwell model, with an EC monolayer on the apical side of the membrane, and supporting cell types in the ‘contact’ and ‘non-contact’ positions on the underside of the membrane and in the basolateral chamber. TEER is measured between electrodes located in each compartment. Permeability is measured by introducing a solute of interest into the apical chamber and measuring the time-dependent concentration in the basolateral chamber. (b) A microfluidic version of the transwell model.