Figure 3.

Dependence of the amperometric responses obtained with the developed biosensor in the absence (N) and in the presence of 100 pM (S) miRNA-21 and the resulting S/N current ratios, with the pre-incubation time of anti-DNA–RNA hybrid antibody and ProtA–PolyHRP40 mixture solution (a), and with the incubation time of b-DNACp-MBs with the mixture solution containing the target miRNA and the anti-DNA–RNA hybrid antibody preincubated for 1 h with the ProtA–PolyHRP40 (b). Error bars estimated at triple of the standard deviation of three replicates.