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Sensitivity obtained for different working conditions checked in the assessment of assay time shortening.
| b-DNACp-MBs-Mixture Solution Incubation Time, min | [Anti DNA–RNA Hybrid Antibody], µg·mL−1 | [ProtA–HRP40], μg·mL−1 | Slope, nA·nM−1 | Sensitivity, % |
|---|---|---|---|---|
| 30 | 2.0 | 2.0 | 55,214 ± 921 | 100 |
| 15 | 2.0 | 2.0 | 34,843 ± 2542 | 63.1 |
| 4.0 | 2.0 | 30,844 ± 4494 | 55.9 | |
| 2.0 | 4.0 | 53,899 ± 659 | 97.6 | |
| 4.0 | 4.0 | 48,862 ± 4269 | 88.5 |
