Table 1.

ICC protocol.

1° Antibody/Counterstain	Specificity	2° Antibody
Anti-mouse Ki67 (1:250; Abcam, Cambridge, UK)	Nuclear protein expressed during proliferation	Alexa Fluor Goat anti-mouse 594 (1:300; Invitrogen, Carlsbad, CA, USA)
Anti-rabbit desmin (1:300; Novusbio, Littleton, CO, USA)	Myoblast	Alexa Fluor Goat anti-mouse 488 (1:300; Invitrogen, USA)
4,6-diamidino-2-phenylindole (1:15,000; DAPI; Molecular Probes, Eugene, OR, USA)	Nuclei	Not applicable