. 2017 Oct 30;18(11):2242. doi: 10.3390/ijms18112242

Table 2.

The equation to evaluate proportion of myoblasts and fibroblasts, growth rate and proliferative potential.

Assay	Staining of Cells	Equation
Proliferative potential	Desmin (Myoblasts) + Ki67 (Proliferating cells) + DAPI (Nucleus) [Desmin negative cells were identified as fibroblasts]	$\frac{T o t a l p r o l i f e r a t i n g m y o b l a s t s o r f i b r o b l a s t s \times 100}{T o t a l c e l l s (D A P I p o s i t i v e)}$
Growth rate of myoblasts and fibroblasts	Desmin (Myoblasts) + DAPI (Nucleus) [Desmin negative cells were identified as fibroblasts]	Growth rate (h⁻¹) = ln (Xa₂/Xa₁)/∆t
Percentage of myoblasts/fibroblasts	Desmin (Myoblasts) + DAPI (Nucleus) [Desmin negative cells were identified as fibroblasts]	$\frac{T o t a l m y o b l a s t s / f i b r o b l a s t s}{T o t a l c e l l s} \times 100$