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. 2017 Oct 26;18(11):2244. doi: 10.3390/ijms18112244

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Construction of three clinical G6PD mutants. (A) The polymerase chain reaction (PCR) amplified product from the first and second round in agarose gel electrophoresis (1%). M: Marker O´GeneRuler 1 kb DNA ladder (Thermo Scientific). Lane 1, G6PD A+ fragment 1; lane 2, G6PD A+ fragment 2; lane 3, amplification of the full-length G6PD G6PD A+; lane 4, G6PD Nefza fragment 1; lane 5, G6PD Nefza fragment 2; lane 6, amplification of the full-length G6PD Nefza; (B) Electropherogram of the single mutant G6PD A+ (A376G, Asn→Asp); (C) Electropherogram of the single mutant G6PD Nefza (T968C, Leu→Pro). In all cases the mutations with corresponding nucleotide and amino acid substitutions are shown.