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Thermal inactivation assays of recombinant G6PD variants in the presence and absence of different concentrations of NADP+. (A) WT G6PD; (B) G6PD A+; (C) G6PD Nefza; (D) G6PD A−. In all cases, 200 ng of total protein was used. Residual activity was expressed as a percentage of the activity for the same sample incubated at 37 °C. The assays were performed in triplicate; standard errors were lower than 5%.
