Figure 8.

Fluorescence emission spectra of the G6PD variants. (A) Intrinsic fluorescence spectra of recombinant human WT G6PD and three clinical variants G6PD A+; Nefza and A− in the absence of NADP⁺; (B) 8-Anilinonaphthalene-1-sulphonate (ANS) accessibility assays of WT G6PD and the three clinical variants in the absence of NADP⁺. Values obtained from buffer containing ANS without protein (open stars) were subtracted from the recordings with protein. The experimental conditions for all the experiments are described in the Materials and Methods section.