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Purification summary of three recombinant human G6PD enzymes.
| G6PD | Total Protein (mg) | Specific Activity (IU·mg−1) | Total Activity (IU) | Yield (%) |
|---|---|---|---|---|
| WT | 4.8 | 224 | 1075 | 61 |
| A+ | 3.6 | 114 | 410 | 43 |
| Nefza | 3.52 | 62 | 218 | 28 |
| A− | 3.53 | 22 | 77 | 24 |
Values are for a typical expression purification experiment; the result varied <10% from batch to batch. The G6PD activity was measured under the standard conditions described in the Materials and Methods.
