Figure 1.
High Performance Liquid Chromatography coupled with Diode Array Detector (HPLC-DAD) chromatogram of aqueous extract from the leaves of Aspidosperma pyrifolium (3 mg/mL). The analysis shows one peak (retention time: 41.18 min) corresponding to rutin (inset shows structure and maximum UV absorption at 256 nm and 353 nm). The separation was performed using a Reverse Phase Luna C-18 column (4.6 mm × 250 mm, 5 μm; Phenomenex). The solvent system used was a gradient of 0.3% acetic acid in water: acetonitrile with a flow rate kept at 1.0 mL min−1. Detection at 340 nm.