Figure 4.

Effect of dose and time-kinetics of Tityus serrulatus venom-induced inflammation. Effect of venom dose on inflammatory response in envenomation-induced peritonitis model in mice. Animals received an intraperitoneal injection of 100 μL of VTs (2.5, 5, 7.5 and 10 μg/animal) and 100 μL (intravenous) of saline. After six hours, mice were euthanized and peritoneal wash and total cell counts were performed (A). Time-kinetics of VTs-induced inflammation and of rutin treatment on the envenomation-induced peritonitis model in mice. Animals received an intraperitoneal injection of 100 μL of VTs (7.5 μg/animal) and were treated with saline or rutin 5 mg/kg (intravenous). Peritoneal wash and total cell counts were performed at indicated time-points after envenomation (B). *** p < 0.001, ** p < 0.01 and * p < 0.05 when compared to the venom group. Data were analyzed using ANOVA followed by Tukey Test. Sal: saline and VTs: Tityus serrulatus venom.