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. 2017 Oct 28;18(11):2265. doi: 10.3390/ijms18112265

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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ASP recovers cytokines production from 5-FU injured bone marrow stromal cells: (a) SDF-1 (also named CXCL12) protein expression in HS-5 cells is detected via confocal laser scanning microscopy and shown from a merged image of FITC-conjugated SDF-1 and DAPI staining of the nuclei in control (top), ASP-treated (the second panel), 5-FU-treated (the third panel) and 5-FU + ASP-treated (bottom) cells (Scale bar = 50 μm); (b) Mean fluorescence intensity of SDF-1 was quantified and is presented as means ± SD; (c) (ELISA) assay was employed to detect the levels of cytokines produced by HS-5 cells. The results are presented as means ± SD. * p < 0.01 vs. control group, # p < 0.01 vs. 5-FU group.