Figure 1.

Conservation and predicted localization of conserved PIN cysteines. (A) Alignment of Arabidopsis PIN protein domains flanking conserved cysteines. Conserved cysteines are displayed in red; (B) Alignment of PIN protein domains flanking conserved cysteines, from representative Embryophyta and charophyte green algae. Conserved cysteines are displayed in red. Lack of indicated amino acid positions denotes incomplete sequences; (C) 2-D model displaying potential membrane conformation of PIN2. Red barrels represent predicted transmembrane helices, separated by loop domains (black lines). According to these predictions, both cysteines are facing the cytoplasm. The positions of Cys-39 in loop 1 and Cys-560 in loop 7 are indicated (yellow circles).