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. 2017 Oct 30;18(11):2279. doi: 10.3390/ijms18112279

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Pharmacological characterization of the PeaTAR1B receptor. (a) Concentration-dependent effects of tyramine on intracellular cAMP levels in HEK 293 (flpTM) cells constitutively expressing the PeaTAR1B-HA receptor and in non-transfected cells. Relative fluorescence (corresponding to the amount of cAMP) is given as the percentage of the value obtained with 1 µM NKH 477 (=100%), a water-soluble forskolin analog. All measurements were performed in the presence of 100 µM isobutylmethylxanthine (IBMX). Data points represent the mean ± SD of eight values (PeaTAR1B-HA) or four values (non-transfected) from a typical experiment; (b) Effects of putative tyramine receptor antagonists on NKH 477-stimulated cAMP production in PeaTAR1B-HA expressing cells. Concentration series of the substances were applied in the presence of 1 µM NKH 477, 10 nM tyramine and 100 µM IBMX. Data represent the mean ± SD of four values from a typical experiment. Determinations for both (a,b) were independently repeated at least three times.

Pharmacological characterization of the PeaTAR1B receptor. (a) Concentration-dependent effects of tyramine on intracellular cAMP levels in HEK 293 (flpTM) cells constitutively expressing the PeaTAR1B-HA receptor and in non-transfected cells. Relative fluorescence (corresponding to the amount of cAMP) is given as the percentage of the value obtained with 1 µM NKH 477 (=100%), a water-soluble forskolin analog. All measurements were performed in the presence of 100 µM isobutylmethylxanthine (IBMX). Data points represent the mean ± SD of eight values (PeaTAR1B-HA) or four values (non-transfected) from a typical experiment; (b) Effects of putative tyramine receptor antagonists on NKH 477-stimulated cAMP production in PeaTAR1B-HA expressing cells. Concentration series of the substances were applied in the presence of 1 µM NKH 477, 10 nM tyramine and 100 µM IBMX. Data represent the mean ± SD of four values from a typical experiment. Determinations for both (a,b) were independently repeated at least three times.