Figure 1.

Generation of tyrosinase-KO mice using Cj-CRISPR/Cas. (A) Schematics of the target locus in tyrosinase. The box outlined with a dotted line indicates the recognition sequence by gRNA, and that outlined with a solid-line indicates PAM. An arrowhead points to the start codon locus of tyrosinase. (B) Some of the offspring by embryo transfer of injected zygotes. (C) Waveform data of deletion mutation and (D) insertion mutation in the obtained pups. PAM and mutation sites are indicated by underline and arrowheads. (E) Suggested mutation patterns in all the offspring.