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. 2017 Oct 31;18(11):2286. doi: 10.3390/ijms18112286

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Zygote-mediated KI at the C-terminal region of Chk2 using Cj-CRISPR/Cas. (A) Schematics of the target sequence. Arrows and underlined sequences indicate the target loci of each gRNAs and its PAM, respectively. The stop codon of Chk2 is shown in bold. The DNA sequence of ssODN is shown in Table S1. (B) Generation efficiencies of genome-modified embryos. (C) Waveform data of the target locus from an embryo modified using gRNA #2. ORF: open reading frame, FLAG: DYKDDDDK epitope tag sequence.