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. 2017 Oct 31;18(11):2289. doi: 10.3390/ijms18112289

Figure 1.

Figure 1

Effect of N-acetylcysteine (NAC) on cell death in 3,5-bis[(2-fluorophenyl)methylene]-4-piperidinone (EF-24)-treated cells. Cells were treated with EF-24 or with EF-24 + 2 mM NAC, as indicated. Incubation proceeded for 24 h prior to appropriate analysis. The experimental points represent mean values from three replicate experiments, with standard deviations. (a) Effect of NAC on nuclear morphology in EF-24 treated cells. Pictures represent typical examples. (b) A quantitative analysis of the effect of NAC on nuclear morphology in EF-24 treated cells. At least 300 cells were examined in each experiment; * denotes significant change in the number of cells with apoptotic nuclei (p < 0.05) between K562 cells treated with EF-24 and cells treated with EF-24 + NAC. (c) Effect of NAC on occurrence of cells in sub G1 phase in response to EF-24 treatment. A representative analysis. (d) A quantitative analysis of the effect of NAC on occurrence of cells in sub G1 phase in response to EF-24 treatment; * denotes significant change in the number of cells sub G1 phase (p < 0.05) between K562 cells treated with EF-24 and cells treated with EF-24 + NAC. (e) Effect of NAC on caspase-3 activation in EF-24-treated cells. After 16 h, cells DEVDase enzymatic activity was determined in cell lysates using Ac-DEVD-AMC; * denotes significant change in DEVDase enzymatic activity (p < 0.05) between K562 cells treated with EF-24 and cells treated with EF-24 + NAC. (f) Effect of NAC on caspase-3 processing in EF-24 treated cells. After 16 h, caspase-3 processing was monitored using western blot analysis. Picture represents a typical example.