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. 2017 Oct 31;18(11):2289. doi: 10.3390/ijms18112289

Figure 5.

Figure 5

EF-24 forms adduct with GSH. (a) MS/MS analysis of the EF-24-GSH adduct: Mass spectrum of the molecular ion of EF-24-GSH adduct (m/z 619.0), result fragment ion [EF-24 + H]+, m/z 312.2, [GSH + H]+, m/z 308.0, and [C9H7NF+H]+, m/z 149.0. (b) Effect of NAC on EF-24-GSH adduct formation. Cells were treated with EF-24 or in the combination with 2 mM NAC for given incubation times. The amount of the EF-24-GSH adduct in cells was determined using LC/MS/MS. The experimental points represent mean values from three replicate experiments, with standard deviations; * denotes significant change in EF-24-GSH adduct content between EF-24 and EF-24 + NAC-treated K562 cells.