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. 2017 Oct 31;18(11):2289. doi: 10.3390/ijms18112289

Figure 6.

Figure 6

Effect of NAC on extracellular and intracellular levels of EF-24. Cells were treated with EF-24 or with EF-24 + 2 mM NAC. At indicated time intervals, extracellular and intracellular levels of EF-24 were determined using LC/MS/MS analysis. (a) Extracellular EF-24 level; * denotes significant change in EF-24 content in growth medium (p < 0.05) between EF-24 and EF-24 + NAC-treated K562 cells. (b) Intracellular EF-24 level; * denotes significant change in intracellular EF-24 content (p < 0.05) between EF-24 and EF-24 + NAC-treated K562 cells. The experimental points represent mean values from three replicate experiments, with standard deviations. (c) MS/MS analysis of EF-24-NAC adduct: Mass spectrum of the molecular ion of [EF-24-NAC-adduct]+, m/z 475.0, result fragment ion [EF-24+H]+, m/z 312.2, and [C9H7NF + H]+, m/z 149.0. (d) Time course of intracellular EF-24-NAC adduct formation. Cells were treated with EF-24 or with EF-24 + NAC for given incubation time. The amount of EF-24-NAC adduct in cells was determined using LC/MS/MS. (e) Time–course of extracellular EF-24-NAC adduct formation. Cells were treated with EF-24 or with EF-24 + NAC for given incubation times. The amount of EF-24-NAC adduct in growth medium was determined using LC/MS/MS. The experimental points represent mean values from three replicate experiments, with standard deviations.