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. 2017 Oct 31;18(11):2289. doi: 10.3390/ijms18112289

Figure 7.

Figure 7

Proapoptotic effects of the EF-24-NAC adduct. EF-24 (50 μM) was mixed with 2 mM NAC in distilled water at ambient temperature and reaction was monitored using LC/MS/MS analysis. When 50% of EF-24 was converted into the EF-24-NAC adduct, reaction was stopped by dilution. Then K562 cells were treated with EF-24 alone or with diluted reaction mixture containing the same amount of EF-24, however, approximately 50% of it was converted into EF-24-NAC adduct. The experimental points represent mean values from three replicate experiments, with standard deviations; * denotes significant change in the number of cells with apoptotic nuclei (p < 0.05) between K562 cells treated with “free” EF-24 and cells treated with a diluted mixture containing the same amount of EF-24 (50% “free” EF-24 + 50% EF-24-NAC adduct).