Figure 7.

Effect of IGF1R/IR antagonists on AR expression in mesenchymal-subtype TNBC cell line BT549. BT549 (mesenchymal-like) cultures were exposed to control (CON), BMS-754807 (BMS; 20 μM) or NVP-AEW807 (NVP; 8 μM) for 12 or 24 h. Total protein was isolated, processed and transferred to PVDF membranes which were probed for AR expression (1:500, Cell Signaling #5153). β-actin (1:2000, Sigma-Adlrich #A1978) was used as a loading control. Western immunoblot representative of three independent experiments.