Figure 3.

Cell viability and aggregate formation of cancer cells printed on glass and NF; Live/Dead assays of HeLa cells printed on the NF and glass. Cells were suspended in culture medium and culture medium containing 0.5% collagen. The live and dead cells are denoted in green and red, respectively (a). Viability of the printed cancer cells (b). Shapes of droplets printed on glass and NF (c); white arrows show protruding cells. Cells were suspended in culture medium and culture medium containing 0.5% collagen. Cell-distributed diameter according to the number of printed droplets (burst shot; 50 times) on glass and the NF (d). All experimental samples were n > 3. * p < 0.01, ** p < 0.005, *** p < 0.001. Unmarked scale bars in Figure 3a and 3c, 50 μm and 100 μm, respectively.