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. 2017 Nov 7;18(11):2348. doi: 10.3390/ijms18112348

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Three-dimensional shapes of cancer microtissues. (a,b) Confocal microscope images of the cancer microtissues. (a) Confocal stacking image (3 μm step; 16 layers) and (b) patterned cancer microtissue. (a,b) Green indicates HPV18, and red indicates F-actin. (c) SEM image (white arrow: fibroblast, red arrow: cancer tissue). (d,e) Frozen section images, (d) mono-cultured sample, and (e) co-cultured sample. Blue indicates Hoechst 33342 (d,e); only cancer cells are stained. Green indicates F-actin; both fibroblast and cancer cells are stained. All experimental samples were n > 3.