Figure 6.

Specific binding activity of VDR to the putative BP3-VDRE sequences by the EMSA non-radioactive method. The biotin 3′ end labelled probe BP3-VDRE (A,B) was incubated with 5 µg of nuclear extract isolated from LIR-MO (A) and HIR-MO (B) both in the presence of 1,25-d (lane 2). For competition experiments, wild-type non-labelled BP3-wtVDRE (A,B) (lanes 3, 4, and 5), and mutated BP3-mVDRE (A) (lanes 5, 6, and 7). Supershift was performed with specific antisera to VDR (A,B) yielding supershifted complexes (lane 7). Lane 1, no nuclear extract.