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. 2017 Nov 7;18(11):2361. doi: 10.3390/ijms18112361

Table 1.

Primers used in the full-length cloning and expression analysis of EuCHIT2.

Use Name Sequence (5′–3′)
RACE EuCHIT2-GSP1 CAGGCCATAGCTAGTGCTGATGTTG
EuCHIT2-GSP2 CCCGCCCGGAACTTCTACACCTACG
Full-length EuCHIT2-XbaI-F GCTCTAGAGCAAATGAGGTTTACCTTGTCCACTCTCC
EuCHIT2-EcoRI-R CGGAATTCCGGACTACTGGGCCTGAACTAAAAGCC
Verification psH-35s-F TCGTCAACATGGTGGAGCACGAC
TEuCHIT2-R CGAATCCGGCGAAAGATCTG
Internal control Actin-F TGGTTAAGGCTGGATTTGCT
Actin-R TGCATCCTTTGACCCATAC
Expression Analysis PR-1a-F ACAGCTCGTGCAGATGTAGGT
PR-1a-R GCTAGGTTTTCGCCGTATTG
COI1-F GTTGTAGCCAGTGAGGGAAATA
COI1-R TTGCCCAGCAAGAGAATAGTAG