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. 2017 Nov 8;18(11):2366. doi: 10.3390/ijms18112366

Figure 1.

Figure 1

A schematic diagram of the PC::G5-tdT reporter allele in the Polr2a locus. The reporter cassette was inserted 3′ of the last exon, without disturbing gene function. Following Cre/loxP-mediated excision of the transcriptional STOP sequence, the CAG promoter drives GCaMP5G and IRES-tdTomato expression in the Cre-expressing cells (e.g., Aif1(Iba1)-IRES-Cre directs expression to the myeloid-monocytic lineage). If red fluorescence is not desirable, the IRES-tdTomato reporter can be independently removed with FLP recombinase using the FRT sites. Modified from [60].