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. 2017 Nov 9;18(11):2380. doi: 10.3390/ijms18112380

Figure 5.

Figure 5

Knockdown of Wnt3a or β-catenin abrogated the fibrin-enhanced plasminogen expression. OCCM-30 cells were transfected with siRNAs against Wnt3a (A) and β-catenin (B). After 48 h of transfection, the protein levels of Wnt3a and β-catenin were detected by immunoblotting and plasminogen mRNA expression was determined by quantitative real-time PCR normalized to GAPDH. Wnt3a was specifically expressed in OCCM30-fibrin rather than other groups (A) but β-catenin expression was determined in OCCM30-TCD and OCCM30-fibrin (B). The knockdown models of Wnt3a and β-catenin had no expressions levels. Each experiment was repeated two times independently. * Significantly different from the OCCM30-TCD groups (p < 0.05), # significantly different from the si-RNA control groups (p < 0.05).